Summary
Type: SPE
Application #: 304660
Catalog#: 730300 - SPE Cartridges, Chromabond, C18 Hydra, 6ml, PP, 1000mg 30/pk
Detection:
Conditions: Column conditioning: 2 x 6 mL dichloromethane – acetone (1:1, v/v) under vacuum, then 6 mL MeOH without vacuum for 3 min, at least 6 mL HPLC-grade water
(Do not let run the column dry!)
Sample aspiration: The prepared sample is passed through the column by vacuum (flow rate 10 mL/min).
Drying: vacuum, 5 min
Elution: 2 x 6 mL dichloromethane – acetone (1:1, v/v)
Evaporation: to dryness with gentle stream of nitrogen
Reconstitution: 0.05 mL acetonitrile – water (1:1, v/v); vortex mixed for 15 s prior HPLC analysis
Authors: Boti, V.I. et al.
Source: J. Chromatogr. A 1146 (2007), 148–156